Scotland's Labour leader Anas Sarwar is to lay down a motion of no confidence in the Scottish government later today, Sky News understands.
This would be separate to the existing no-confidence motion which is in Humza Yousaf as first minister.
If the motion of no confidence in Mr Yousaf passes next week, he would not be compelled to resign - although failing to hold the confidence of the majority of parliament would put significant pressure on his position.
However, if there is a vote of no confidence in the government the first minister and ministers would be forced to step down.
Parliament would have 28 days to choose a new first minister or call an election.
"I think it is now a matter of when, not if Humza Yousaf resigns as first minister," Mr Sarwar told LBC.
"But I think it would be completely untenable for the SNP to presume they can impose another unelected first minister in Scotland.
"Ultimately I think it is the people of Scotland that should decide who leads this country and not just a small group of SNP members."